The KNOTTIN database

Chemical synthesis & recombinant protein expression

  • Since knottins are considered as interesting leads or scaffolds in drug design, it is necessary that these miniproteins can be obtained easily.
  • As they are very small proteins, many knottins were obtained by chemical synthesis.
  • Several linear and cyclic knottins were also obtained using recombinant technologies.
  • Main techniques used to prepare knottins (including cyclic knottins) are outlined below.

Knottins can be obtained either

Synthesis

Expression

Protease inhibitors

Cyclotides

Toxins

Others

Others

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